The Anti-TPO ELISA kit is intended for quantitative determination of autoantibodies against thyroid peroxidase in human serum.
TPO is a key enzyme in the biosynthesis of thyroid hormones. Autoantibodies against thyroid peroxidase (Anti-TPO) are mainly IgG and less frequently IgM. In healthy individuals concentrations of Anti-TPO in serum may reach 30 U/mL. Anti-TPO levels exceeding this limit are the sign of an autoimmune process that usually leads to thyroid dysfunction, mainly due to reduced thyroxin secretion. Determination of Anti-TPO antibodies in serum is used for diagnosis of autoimmune thyroid diseases like idiopatic mixoedema, Hashimoto thyroiditis and Graves disease.
| Kit volume, wells (including controls) | 96 |
| Analytical sensitivity, U/mL | 4 |
| Range of evaluated concentrations, U/mL | 4–500 |
| Incubations | 30 min + 30 min + 10 min at 37°Cor
30 min + 30 min at 37°C + 15-30 min at RT |
| Shelf life, months | 12 |